Anti-CKMT1B Antibody

This rabbit polyclonal antibody from Antibodies.com is raised against CKMT1B (creatine kinase, mitochondrial) and reacts with human, mouse and rat samples. It is supplied as an unconjugated IgG in liquid form, produced by affinity purification from a rabbit host.

The immunogen is a recombinant fusion protein corresponding to amino acids 1-85 of human CKMT1B (NP_066270.1), and the target protein has a molecular weight of 42 kDa. The product is formulated in phosphate buffered saline, pH 7.3, with 50% glycerol and 0.01% thiomersal. It is shipped at 4°C and should be aliquoted and stored at -20°C, with freeze/thaw cycles to be avoided.

Validated for western blot (WB), immunocytochemistry/immunofluorescence (ICC/IF) and immunoprecipitation (IP), the antibody is supported by four scientific images. Recommended dilutions are WB: 1:500-1:2,000, ICC/IF: 1:50-1:100, and IP: 1:500-1:1,000. Representative data include western blot analysis of various cell line extracts, immunofluorescence on NIH-3T3 and U-2 OS cells at 1:100 dilution, and immunoprecipitation of 600 µg of mouse brain extract using 3 µg of antibody.