Anti-CHRNA2 Antibody

The CHRNA2 gene encodes the alpha-2 subunit of the neuronal nicotinic acetylcholine receptor, a pentameric ligand-gated ion channel expressed throughout the central and peripheral nervous system. Variants in CHRNA2 have been linked to autosomal dominant nocturnal frontal lobe epilepsy (ADNFLE), and altered receptor expression is actively studied in the context of nicotine dependence and cholinergic neurotransmission research. This Antibodies.com rabbit polyclonal reagent is raised against CHRNA2 and supplied as an unconjugated 100 µL liquid aliquot suitable for Western blot and immunohistochemistry workflows on human samples. The polyclonal format provides recognition of multiple epitopes across the target, which is helpful when working with post-translationally modified receptor subunits or truncated variants. Researchers investigating nAChR subunit composition, synaptic signaling, or epilepsy-associated channelopathy models use anti-CHRNA2 antibodies to map receptor distribution and to confirm expression in transfected cell lines and primary neuronal cultures. For Western blot, the antibody is applied to membrane-bound preparations from brain-derived lysates, while immunohistochemistry supports localization studies in fixed tissue sections. The unconjugated format allows pairing with a preferred secondary detection system, whether HRP, biotin-streptavidin, or fluorescent labels matched to existing imaging platforms. Standard short-term storage at refrigerated temperatures preserves activity between uses, and small aliquots help limit repeated freeze-thaw cycles that can degrade polyclonal preparations.