Anti-CHRFAM7A Antibody

Raised against a synthetic peptide spanning the junction between CHRNA7 (exons 5–10) and FAM7A (exons A–E), this affinity-purified rabbit polyclonal reagent from Antibodies.com detects endogenous levels of the chimeric dupα7 protein encoded by the partially duplicated CHRFAM7A locus on chromosome 15. Because CHRFAM7A shares extensive amino-acid identity with the canonical α7 nicotinic receptor subunit, the polyclonal format offers broader epitope coverage than a monoclonal reagent — useful for resolving low-abundance dupα7 from full-length CHRNA7 in tissue lysates and fixed sections where the two species often co-express.

Supplied as a 100 µl liquid aliquot of unconjugated rabbit IgG formulated in PBS (pH 7.3) with 0.05% sodium azide and 50% glycerol, the antibody leaves the choice of secondary detection chemistry (HRP, biotin, or fluorophore conjugates) to the end user. Validated applications are Western blot and immunohistochemistry, with reactivity confirmed against endogenous CHRFAM7A in human, mouse, and rat samples. Researchers probing the cholinergic anti-inflammatory pathway, α7nAChR stoichiometry in neural and immune tissue, or the contribution of duplicated CHRFAM7A alleles to inflammatory and neuropsychiatric disease can apply this reagent to distinguish the duplicated gene product from CHRNA7 — a distinction relevant because dupα7 acts as a dominant-negative regulator of α7nAChR currents and is itself down-regulated by LPS, IL-1β, and nicotine in human macrophages.

For handling, aliquot the antibody upon arrival to limit freeze-thaw cycles and store at -20°C; the reagent may be refrigerated for routine short-term use. As a research use only reagent, it supports neuroscience, immunology, and inflammation laboratories that need to differentiate the fused CHRFAM7A gene product from its parental CHRNA7 subunit across species and tissue types.