Anti-CHRAC1 Antibody

Raised in rabbit as a polyclonal reagent against the chromatin accessibility factor CHRAC1, this unconjugated primary antibody is supplied by Antibodies.com at 1 mg/ml in phosphate buffered saline containing 0.05% sodium azide, at approximately pH 7.3. CHRAC1 is the small histone-fold subunit of the CHRAC chromatin accessibility complex, where it partners with ACF1, the ATPase SNF2H, and CHRAC-17 to support nucleosome sliding, chromatin assembly during DNA replication, and transcription-related remodeling at promoter and enhancer regions.

Validated for Western blot on lysates of human, mouse, and rat origin, the reagent recognizes endogenous CHRAC1 in standard immunoblotting workflows. The 100 µl volume supports multiple probing rounds when diluted to working concentration in blocking buffer, and the sodium azide preservative permits short-term benchtop handling. Refrigerate in the original vial and protect from light to preserve activity across routine use.

Typical applications include probing CHRAC1 levels in chromatin-enriched fractions or whole-cell extracts, comparing subunit incorporation within the CHRAC complex, and tracking chromatin remodeler abundance across different cell states. Pair with HRP- or fluorophore-conjugated secondary antibodies for chemiluminescent or fluorescent detection on PVDF or nitrocellulose membranes.