Anti-Chk2 (S62) Antibody

This rabbit polyclonal antibody from Antibodies.com specifically recognizes Checkpoint Kinase 2 (Chk2) phosphorylated at Serine 62. Chk2 is a central effector of the ATM-dependent DNA damage response pathway, where phosphorylation events at sites including S62 contribute to its activation following genotoxic stress, leading to downstream phosphorylation of substrates such as p53, BRCA1, and CDC25A and the coordination of cell cycle arrest, DNA repair, or apoptosis.

Supplied as 50 µl of unconjugated antibody at 1 mg/ml in a vial of phosphate buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.2, the reagent is validated for Western blot and immunohistochemistry applications. The antibody has been confirmed to react with Human, Mouse, and Rat samples, supporting translational studies across these species in both cell culture models and fixed tissue specimens.

Phospho-specific reagents targeting Chk2 at Serine 62 are commonly used to monitor checkpoint activation in radiation biology, cancer cell signaling, and chemotherapeutic response research. To preserve activity, aliquot the antibody upon arrival to limit freeze-thaw cycles during long-term storage.