Anti-Chk2 (S379) Antibody

Checkpoint kinase 2 phosphorylated at serine 379 is the target of this rabbit polyclonal reagent from Antibodies.com, an unconjugated preparation validated for Western blot and immunohistochemistry on human, mouse, and rat samples. Supplied as 50 µl at approximately 1 mg/ml in phosphate buffered saline with 0.05% sodium azide at pH 7.2, it supports studies of the DNA damage response where ATM-mediated signaling through Chk2 must be distinguished from total Chk2 levels.

Common applications include tracking Chk2 activation kinetics after genotoxic insult in cultured cells, mapping phospho-Chk2 distribution in formalin-fixed tumor biopsies by IHC, and comparing baseline versus induced signaling in matched normal and diseased tissue lysates. For best results, aliquot upon first thaw and store the working stock at -20 °C; repeated freeze-thaw cycles of the parent vial should be avoided to preserve binding activity.