Anti-Chk2 (phospho-T432) Antibody

The Antibodies.com rabbit polyclonal antibody targets Chk2 phosphorylated at threonine 432, a post-translational modification triggered by DNA double-strand breaks and ATM kinase activation during the cellular response to genotoxic stress. Supplied as a 100 µl vial at 1 mg/ml in phosphate-buffered saline (approximately pH 7.2) with 0.05% sodium azide as a preservative, this unconjugated primary antibody is validated for western blot detection of activated Chk2 in human and rat samples. For best results, store refrigerated and avoid repeated freeze-thaw cycles to preserve antibody activity. Researchers investigating DNA damage response pathways, p53-mediated tumor suppression, and cell-cycle checkpoint control rely on this reagent to probe Chk2 kinase activity following exposure to ionizing radiation, topoisomerase inhibitors, and other genotoxic agents. The polyclonal format provides broad recognition of the phospho-T432 epitope, supporting reproducible western blot detection in whole-cell lysates, nuclear extracts, and tumor tissue homogenates from both species.