Anti-Chk2 Antibody

Chk2 (Checkpoint Kinase 2) functions as a critical effector kinase in the ATM-mediated DNA damage response pathway, becoming phosphorylated at Thr68 following double-strand DNA breaks. This rabbit polyclonal antibody from Antibodies.com is raised against Chk2 and supplied as a 100 µl unconjugated reagent for laboratory research use only.

The antibody has been validated across Western blot, immunohistochemistry, and ELISA workflows, supporting detection of endogenous Chk2 in Human, Mouse, and Rat samples. Its polyclonal format enables recognition of multiple epitopes on the target, which is useful when monitoring changes in total Chk2 expression or analyzing upstream DDR signaling events in cell lysates and tissue sections.

Applications include studies of genomic stability, G1/S and G2/M cell cycle checkpoint regulation, apoptosis induction following genotoxic stress, and tumor suppressor pathway analysis involving the ATM/Chk2/p53 cascade. The unconjugated format offers flexibility for downstream custom labeling with fluorophores, biotin, or enzymes to suit specific experimental designs in cancer biology, radiobiology, and DNA repair research.