Anti-Chk2 (Ab-68) Antibody

Chk2 (Checkpoint kinase 2) is a serine/threonine protein kinase central to the DNA damage response pathway, where it becomes phosphorylated by ATM following double-strand breaks and coordinates cell cycle arrest through downstream substrates including p53, Cdc25A, Cdc25C, and BRCA1. Antibodies.com offers this rabbit polyclonal antibody raised against a synthetic peptide surrounding residue 68 of the human Chk2 sequence, supplied as 100 µl of unconjugated antisera for Western blot and immunohistochemistry on human samples. Because Chk2 is mutated or lost in Li-Fraumeni syndrome and a range of sporadic cancers, this reagent is commonly used to probe checkpoint integrity, ATM-Chk2-p53 signaling, and genomic instability across cultured cells and archived tissue. The polyclonal format recognises multiple epitopes on the target, which is useful for capturing both the latent and ATM-phosphorylated (Thr68) forms of Chk2 that mark active checkpoint signaling. Researchers working on DNA repair, tumour suppressor biology, and radio- or chemo-sensitization studies can rely on this antibody to confirm expression levels and activation status of Chk2 in their experimental systems.