Anti-Chk1 (phospho-S286) Antibody

Phosphorylation of checkpoint kinase 1 at serine 286 (Chk1 phospho-S286) is a post-translational modification studied in the cellular DNA damage response, where Chk1 coordinates cell-cycle arrest with DNA repair machinery. This unconjugated rabbit polyclonal antibody from Antibodies.com is raised against that phospho-epitope and is applied in Western blot detection of the modified kinase. The reagent recognizes the target in human, mouse, and rat samples, supporting comparative studies of checkpoint signaling in cell-line and tissue models.

Supplied as 100 µl at 1 mg/ml in phosphate buffered saline (PBS) with 0.05% sodium azide at approximately pH 7.2, the antibody ships in a vial ready to dilute into standard blocking buffers. Refrigerate for storage and protect from light; the sodium azide acts as a preservative. Investigators examining ATM/ATR pathway activity, S-phase checkpoint enforcement, or replication stress responses can monitor Chk1 phosphorylation status with this reagent following genotoxic insult.