Anti-Chk1 (P351) Antibody

Distributed through Antibodies.com, this rabbit polyclonal reagent targets Chk1 (Checkpoint Kinase 1, CHEK1) at the P351 region and is supplied as a 50 µl aliquot at 1 mg/ml in phosphate buffered saline (PBS) with 0.05% sodium azide, approximately pH 7.2. The unconjugated antibody is affinity-purified from rabbit antiserum using an epitope-specific immunogen, and the purity is greater than 95% as assessed by SDS-PAGE, yielding an IgG polyclonal preparation of host origin Rabbit. Validated reactivity covers Human, Mouse and Rat samples, and the antibody has been specifically tested in immunohistochemistry (IHC) at the recommended working dilution of 1:50–1:200 on paraffin-embedded tissue, where it produces clear cytoplasmic and nuclear staining of CHK1 with an observed molecular weight near 56 kDa. For short-term use, vials should be kept at 4 °C, while long-term storage is recommended at –20 °C in single-use aliquots to avoid repeated freeze–thaw cycles. Chk1 functions as an essential component of the G2 DNA damage checkpoint, phosphorylating Cdc25C (and in vitro Wee1) to delay mitotic entry after genotoxic stress, so this reagent is well suited to studies of cell cycle control, checkpoint signalling and DNA damage response pathways in cancer and cell biology research. As with all Antibodies.com primary antibodies, the product is supplied for research use only and is not intended for diagnostic or therapeutic applications.