Anti-Chk1 (P351) Antibody

Chk1 (Checkpoint Kinase 1) is a serine/threonine protein kinase that operates as a central effector of the DNA damage response, mediating S-phase and G2/M cell cycle arrest to preserve genomic integrity following replication stress or DNA lesions. This rabbit polyclonal antibody from Antibodies.com targets Chk1 at the region designated P351 and is validated for immunohistochemistry (IHC) on human, mouse, and rat samples.

Supplied as 100 µl at a concentration of 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.2, the unconjugated reagent can be paired with HRP, biotin, or fluorescently labeled secondary antibodies depending on the detection chemistry chosen. The polyclonal format recognizes multiple epitopes on Chk1, which often translates into robust signal in formalin-fixed paraffin-embedded (FFPE) tissue sections, while the broad species reactivity makes this reagent a practical option for comparative studies between rodent disease models and human specimens.

Investigators studying replication fork stability, ATR/Chk1 signaling, oncogene-induced replication stress, or the response to DNA-damaging chemotherapeutics can apply this antibody to FFPE blocks and cryosections to localize Chk1 within tissue architecture. Aliquot the vial upon arrival and refrigerate for routine use; for longer-term storage, freeze and limit freeze-thaw cycles to preserve antibody integrity across repeated experiments.