Anti-Chk1 Antibody

Offered through Antibodies.com, this affinity-purified polyclonal reagent is raised in rabbit against the Chk1 checkpoint kinase (also known as CHEK1, CHK1 checkpoint homolog, or serine/threonine-protein kinase Chk1) and is supplied as a 50 µL liquid formulation at 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA, and 0.02% sodium azide. The immunogen is a synthesized peptide derived from the human Chk1 sequence around the non-phosphorylation site of Ser345, allowing the antiserum to detect endogenous levels of the target across human, mouse, and rat samples. Validated applications include Western Blot, Immunohistochemistry, and ELISA, with published WB data on HT29 cell lysates confirming performance for cell-cycle and DNA-damage response research. The antibody is unconjugated and provided at a working concentration suitable for standard immunodetection protocols. For handling, vials are shipped at 4°C and should be aliquoted upon arrival and stored at -20°C, with repeated freeze/thaw cycles avoided to preserve activity. Researchers studying checkpoint signaling, genomic stability, or kinase phosphorylation dynamics will find this reagent a reliable tool for probing Chk1 expression in both cultured cells and tissue sections.