Anti-Chk1 Antibody

The Chk1 (Checkpoint Kinase 1) protein is a serine/threonine kinase that operates at the core of the DNA damage response, mediating S-phase and G2/M cell cycle arrest following genotoxic stress. This unconjugated polyclonal reagent from Antibodies.com is raised in rabbit and validated for Western blot (WB), immunohistochemistry (IHC), and ELISA workflows, supporting research into checkpoint signaling, replication fork stability, and genome maintenance.

The antibody recognizes Chk1 across human, mouse, and rat samples, enabling cross-species investigation of ATR-Chk1-CDC25 signaling, DNA repair pathways, and cellular responses to chemotherapeutic agents. Supplied as 100 µl of unconjugated polyclonal antiserum, it supports immunoblotting of total Chk1 expression, tissue-section localization by IHC, and ELISA-based quantification from cell lysates and tissue extracts. Applications include probing kinase activity, downstream phosphorylation events, and pathway disruption in DNA damage response models used in oncology and cell cycle research.