Anti-CHEK2 (phospho S516) Antibody

This rabbit polyclonal IgG antibody from Antibodies.com specifically recognizes human CHEK2 only when phosphorylated at serine 516, a residue modified by ATM kinase in response to DNA double-strand breaks. Phospho-S516 has been used as a marker of active checkpoint signaling following genotoxic stress, including ionizing radiation and topoisomerase inhibitor exposure. The unconjugated reagent is supplied as 50 µl of polyclonal antiserum and is validated for Western blot detection of endogenous phosphorylated CHEK2 in human cell lysates. Because the epitope depends on phosphorylation at this site, sample preparation typically includes phosphatase inhibitor cocktails to preserve the modification during lysis. Researchers studying the DNA damage response, cell cycle checkpoints, or tumor suppressor pathways can use this reagent to monitor CHEK2 activation kinetics and downstream substrate phosphorylation events in their experimental workflows.