Anti-CHEK2 (phospho S516) Antibody

This rabbit polyclonal IgG antibody from Antibodies.com selectively detects human CHEK2 (Checkpoint Kinase 2) only when phosphorylated at serine 516, a regulatory site targeted during the DNA damage response that follows double-strand breaks. Raised in rabbit and supplied as an unconjugated polyclonal reagent in a 200 µl vial, the antibody is intended for Western blot analysis of cell lysates and is reactive with human samples. Researchers use it to monitor activation of the CHK2 tumor-suppressor pathway, distinguish phosphorylated CHK2 from total CHK2 in signaling studies, and characterize ATM-dependent and autophosphorylation events at the S516 site. The reagent is IgG-based and unconjugated, giving users flexibility to pair it with secondary antibodies matched to their preferred Western blot detection chemistry.