Anti-CHD4 Antibody

This rabbit polyclonal antibody targets CHD4 (Chromodomain Helicase DNA-binding protein 4), the catalytic ATPase subunit of the nucleosome remodeling and deacetylase (NuRD) complex. CHD4 remodels chromatin in an ATP-dependent manner, mobilizes nucleosomes, and recruits HDAC activity to regulatory regions, influencing transcriptional repression, DNA damage signaling, and cell fate decisions in dividing and post-mitotic cells.

The reagent is supplied as 100 µl of an unconjugated, affinity-purified antibody at 1 mg/ml in phosphate-buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.3. It has been validated for Western blot on lysates of human, rat, and mouse origin, supporting cross-species work in chromatin biology, cancer research, and DNA damage response studies.

For best results, aliquot the antibody upon arrival and store at -20°C in a frost-free freezer; avoid repeated freeze-thaw cycles. For short-term use, keep refrigerated and protect from light. The polyclonal format provides multi-epitope recognition of full-length CHD4, producing a band near 218 kDa on Western blot under standard reducing conditions. This Antibodies.com primary antibody is intended for research use only.