Anti-CHAT Antibody

Choline Acetyltransferase (CHAT) is the enzyme that catalyzes acetylcholine synthesis from choline and acetyl-CoA, and it serves as the canonical marker for cholinergic neurons. This Rabbit polyclonal antibody is supplied as 100 µl of unconjugated antisera and is validated for Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF) detection in Human, Mouse, and Rat samples. Investigators working on basal forebrain cholinergic projections, neuromuscular junctions, or the enteric nervous system rely on Anti-CHAT reagents to resolve cholinergic cell bodies and fiber tracts in tissue sections and to detect the roughly 70 kDa enzyme band by immunoblotting. The unconjugated format offers flexibility for downstream labeling with fluorophores, HRP, or biotin chosen by the end user, supporting custom detection schemes such as tyramide signal amplification, double-labeling with other neuronal markers, or pre-embedding immuno-electron microscopy. Because CHAT is a low-abundance intracellular target, amplification-based chromogenic IHC and standard IF protocols both perform well with this reagent. The polyclonal nature of the antibody broadens epitope coverage, which can be advantageous when probing formalin-fixed paraffin-embedded tissue where antigen masking is a concern. Across neuroscience, neurodegenerative disease models, and developmental biology workflows, this Antibodies.com reagent provides a dependable tool for mapping cholinergic circuitry.