Anti-CHAF1B Antibody

CHAF1B (chromatin assembly factor 1 subunit B, also called CAF-1 p60) is the p60 subunit of the CAF-1 histone chaperone complex that deposits newly synthesized histones H3 and H4 onto replicating DNA during S phase and supports nucleosome assembly after DNA repair. This Rabbit polyclonal reagent from Antibodies.com targets the human CHAF1B protein and is supplied as a 100 µL liquid aliquot in a vial. The unconjugated antibody is validated for western blot (WB) and immunofluorescence (IF) workflows, making it useful for studies of replication-coupled chromatin assembly, heterochromatin maintenance at pericentric regions, and the DNA damage response. CHAF1B expression has been investigated as a prognostic indicator in several malignancies, including hepatocellular carcinoma and breast cancer, where elevated levels often correlate with more aggressive tumor behavior, so a sensitive anti-CHAF1B reagent is helpful for tumor biology work as well as basic chromatin research. For daily use, store the vial refrigerated at 4 °C and aliquot into smaller portions if long-term storage is planned; avoid repeated freeze-thaw cycles to preserve binding activity.