Anti-cGKII Antibody

Raised in rabbit against the cGMP-dependent protein kinase II target, this polyclonal reagent from Antibodies.com is supplied as 100 µl of unconjugated antisera validated for Western blot, immunohistochemistry, and ELISA workflows. cGKII, encoded by the PRKG2 gene, is a serine/threonine kinase activated by cyclic GMP that mediates downstream phosphorylation in nitric oxide and natriuretic peptide signaling cascades, with documented roles in chondrocyte proliferation, long-term potentiation, and intestinal anion secretion.

Reactivity has been confirmed in human, mouse, and rat samples, supporting use in translational panels that span rodent disease models and human tissue archives. Investigators studying smooth muscle relaxation, renal sodium handling, or hippocampal plasticity commonly deploy this reagent to monitor PRKG2 expression across developmental stages or following pharmacological manipulation of intracellular cGMP levels.

For long-term use, the antibody should be stored frozen at -20 °C and partitioned into aliquots to limit repeated freeze-thaw cycles of the original vial. The unconjugated format provides flexibility to conjugate the antibody to fluorophores, biotin, or horseradish peroxidase depending on the chosen detection platform, and the polyclonal composition improves the probability of recognizing multiple epitopes on the target protein, which is useful when working with denatured lysates in Western blot or fixed, paraffin-embedded specimens in immunohistochemistry.