Anti-CFC1 Antibody

This Rabbit polyclonal antibody from Antibodies.com targets CFC1 (Cryptic Family EFCAB3), a GPI-anchored membrane co-receptor essential for Nodal signaling during vertebrate embryonic patterning. CFC1 cooperates with type II activin receptors such as ACVR2B to enable Nodal-mediated phosphorylation of SMAD2 and SMAD3, a signaling axis that governs left-right axis determination, mesendoderm induction, and early cardiac looping morphogenesis. Loss-of-function variants in CFC1 are associated with autosomal dominant heterotaxy syndrome and a spectrum of congenital heart defects, including transposition of the great arteries, double-outlet right ventricle, and atrial isomerism, which makes a well-characterized detection reagent valuable for translational cardiovascular and developmental biology research.

Supplied as 100 µl of unconjugated polyclonal antiserum raised in Rabbit, the reagent is validated for Western blot and immunohistochemistry workflows on Human samples. The unconjugated format offers flexibility for downstream labeling with fluorophores, biotin, or reporter enzymes to suit a custom assay design. For routine use, store at -20 °C in working aliquots to limit freeze-thaw cycles and preserve binding activity over the shelf life of the stock.