Anti-CERS4 Antibody

Raised in rabbit against the ceramide synthase 4 (CERS4) protein, this unconjugated polyclonal antibody from Antibodies.com is supplied as a 100 µl liquid aliquot and supports detection of the endogenous human target in Western blot and immunohistochemistry workflows. CERS4, also called LASS4, is an endoplasmic reticulum membrane enzyme that catalyzes the N-acylation of a sphingoid base to produce dihydroceramide, a key step in the de novo sphingolipid biosynthesis pathway. The reagent is validated for human samples and recognizes the full-length CERS4 polypeptide encoded by the CERS4 gene on chromosome 19p13.2.

For Western blot, the antibody is typically applied at a working dilution suitable for chemiluminescent or fluorescent readout of the approximately 46 kDa CERS4 band in cell lysates and tissue homogenates. In immunohistochemistry, it performs on formalin-fixed, paraffin-embedded sections using standard antigen retrieval protocols, allowing visualization of CERS4 expression patterns in tissues where sphingolipid metabolism is under study. Aliquot the antibody upon arrival to limit freeze-thaw cycles; short-term storage at 4 °C is acceptable for immediate use, while long-term stability is best preserved at -20 °C or below, protected from light.

Provided as a single-use research reagent, this polyclonal antibody from Antibodies.com offers an affinity-purified option for laboratories investigating ceramide homeostasis, lipid signaling, and the contribution of sphingolipid intermediates to cell stress, apoptosis, and metabolic disease research.