Anti-CDKN1A (phospho T145) Antibody

CDKN1A encodes the cyclin-dependent kinase inhibitor p21, a key mediator of p53-driven G1/S checkpoint arrest following DNA damage. Threonine 145 phosphorylation represents a stress-responsive modification that modulates subcellular localization of p21 and influences crosstalk with pro-survival kinase signaling pathways, including AKT-dependent survival cascades.

This unconjugated rabbit polyclonal reagent from Antibodies.com is raised against the phospho-T145 epitope of human p21 and is validated for immunohistochemistry on formalin-fixed tissue sections. The IgG isotype formulation is supplied as a 100µl aliquot, ready to use in chromogenic detection workflows in apoptosis, cell-cycle, and tumor biology research.

Reactivity is confirmed against the human antigen, with application notes supporting IHC on paraffin-embedded sections. The reagent enables researchers to distinguish the activated, phosphorylated form of p21 from total CDKN1A expression in studies of genotoxic stress, cellular senescence, and chemotherapy response, providing a specific read-out of pathway activation rather than baseline protein levels.