Anti-CDK6 (Ab-24) Antibody

Manufactured by Antibodies.com as a primary antibody for cell-cycle research, this rabbit polyclonal reagent targets the cyclin-dependent kinase 6 (CDK6) epitope surrounding residues 22–26 (G-A-Y-G-K), enabling detection of endogenous total CDK6 protein. Supplied at 50 µl per vial with a concentration of 1.0 mg/mL, the unconjugated antibody is validated for Western blot (WB) and immunohistochemistry (IHC) workflows, and has been tested on lysates from HT29 and 293 cells as well as paraffin-embedded human colon carcinoma sections. The host species is rabbit and the clonality is polyclonal, making it suitable for broad epitope recognition across the conserved kinase region. Cross-reactivity has been confirmed in human, mouse, and rat samples, supporting studies ranging from human cancer cell-line panels to rodent tissue models. The antibody is affinity-purified using an epitope-specific peptide after immunisation with the immunogen–KLH conjugate, then formulated in phosphate-buffered saline (pH 7.4) containing 150 mM NaCl, 0.02% sodium azide and 50% glycerol to preserve activity during shipping and short-term storage. For long-term storage the supplier recommends shipping at 4 °C, aliquoting on arrival, and freezing at –20 °C while avoiding repeated freeze/thaw cycles. Intended strictly for research use only and not for diagnostic or therapeutic applications, this Antibodies.com reagent provides a reliable tool for investigating CDK6-mediated regulation of the G1/S cell-cycle transition, including its interactions with D-type cyclins in proliferating and transformed cells.