Anti-CDK4 (N-term) Antibody

Cyclin-dependent kinase 4 (CDK4) governs the G1-to-S checkpoint of the eukaryotic cell cycle by partnering with D-type cyclins to phosphorylate the retinoblastoma protein, releasing E2F transcription factors and committing the cell to division. This unconjugated mouse monoclonal IgG1 reagent from Antibodies.com binds the N-terminal region of CDK4 and is validated for Western blot and immunocytochemistry on lysates and cultured cells of human, rabbit, canine, rat, and mouse origin. The antibody is supplied as 100 µl of liquid, allowing multiple blotting membranes or coverslip preparations from a single vial, and should be stored frozen in aliquots to protect from light and limit freeze-thaw cycles. Researchers investigating melanoma, glioblastoma, and other malignancies in which the CDK4–Rb axis is dysregulated, as well as those mapping proliferation kinetics in normal and transformed cell lines, will find this N-terminal epitope useful for detecting endogenous CDK4 protein by Western blot or immunocytochemistry workflows.