Anti-CDK4 Antibody

Manufactured by Antibodies.com, this affinity-purified reagent is a Rabbit polyclonal antibody raised against CDK4 (Cyclin-Dependent Kinase 4), validated for use in Western Blotting (WB) and Immunohistochemistry (IHC) on human samples. The antibody detects endogenous levels of total CDK4 protein and was generated by immunizing rabbits with a synthetic peptide corresponding to the sequence around amino acids 295–299 (L-H-K-D-E) of human CDK4, followed by affinity-chromatography purification on an epitope-specific peptide column. It is supplied at a concentration of 1.0 mg/mL in 50 µl of phosphate-buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol, and is unconjugated for downstream flexibility in labeling and detection workflows. As a polyclonal reagent raised in Rabbit, it offers broad epitope coverage and is suitable for researchers studying cell-cycle regulation, G1/S progression, and CDK4-RB pathway signaling in human tissues and cell lines. The product is shipped at 4 °C; upon arrival it should be aliquoted and stored at –20 °C, with freeze/thaw cycles avoided to preserve antibody integrity. Intended for research use only, this primary antibody is dispatched from St. Louis, MO, with a typical lead time of 6–9 business days, making it a reliable choice for laboratories requiring consistent, batch-validated performance across WB and IHC applications.