Anti-CDK4 Antibody

Rabbit polyclonal antibody to CDK4, offered by Antibodies.com as a primary antibody for research use. The reagent is raised in rabbit, is of the IgG isotype and is supplied unconjugated. It is validated for Western blot (WB), immunohistochemistry (IHC) and immunofluorescence (IF), with recommended WB dilutions of 1:500–1:1,000. Sources differ on species coverage: the product attributes list reactivity with human, mouse and rat, while manufacturer documentation describes reactivity with human samples.

The immunogen corresponds to human CDK4 (NP_000066.1); one source describes a synthetic peptide covering amino acids 200–303 of human CDK4, and another describes a recombinant fusion protein containing amino acids 1–303 of human CDK4. The antibody is affinity purified and supplied as a liquid at lot-specific concentration. Reported molecular weight of the target is 34 kDa. Formulation is phosphate buffered saline (pH 7.3) containing 50% glycerol and 0.01% thiomersal. The product is shipped at 4°C; on receipt it should be aliquoted and stored at -20°C, avoiding freeze/thaw cycles.