Anti-Cdk1/Cdc2 (phospho-Y15) Antibody

This rabbit polyclonal antibody from Antibodies.com is raised against Cdk1 (also known as Cdc2) when phosphorylated at tyrosine 15, an inhibitory modification that holds the kinase inactive during interphase and is removed by Cdc25 phosphatases to drive mitotic entry. The reagent is supplied as 100 µl of polyclonal IgG at 1 mg/ml in phosphate buffered saline with 0.05% sodium azide, pH approximately 7.2, ready for use in western blot and immunohistochemistry workflows on human, mouse, and rat samples. Researchers studying G2/M checkpoint control, the DNA damage response, and the regulation of Wee1- and Myt1-mediated inhibitory phosphorylation commonly use phospho-Y15 Cdk1 detection to monitor checkpoint activation, premature mitotic entry, and the efficacy of small-molecule Wee1 inhibitors in cancer cell models. The unconjugated format supports flexible secondary antibody pairing for immunoblotting of cell lysates and IHC staining of paraffin or frozen tissue sections.