Anti-CDH1 Antibody [Assigned #A5513]

Mouse monoclonal IgG antibody recognizing CDH1 (E-cadherin), a calcium-dependent transmembrane glycoprotein that mediates homotypic adhesion between epithelial cells. This unconjugated primary reagent is supplied as a 50 µL aliquot and is validated for immunohistochemistry on formalin-fixed, paraffin-embedded human tissue. E-cadherin immunolabeling is widely used in surgical pathology to confirm epithelial differentiation, to distinguish lobular carcinoma from ductal lesions of the breast, and to assess epithelial-mesenchymal transition status in carcinoma specimens. The clone is raised in mouse and is of the IgG isotype, allowing detection with standard anti-mouse secondary detection systems using HRP or alkaline phosphatase with chromogenic substrates. Researchers and diagnostic laboratories use this reagent to localize CDH1 at the plasma membrane of glandular and surface epithelia, where loss of membranous expression can indicate lobular breast carcinoma or hereditary diffuse gastric carcinoma. The vial from Antibodies.com should be handled under refrigerated conditions, protected from light, and aliquoted to limit freeze-thaw cycles, supporting reproducible immunostaining across runs.