Anti-CDH1 Antibody [Assigned #A5513]

CDH1 encodes E-cadherin, a calcium-dependent transmembrane glycoprotein that mediates homophilic adhesion between epithelial cells and maintains tissue architecture. The assigned clone #A5513 is a mouse monoclonal IgG antibody recognizing the human CDH1 protein, supplied by Antibodies.com for immunohistochemistry (IHC) on formalin-fixed paraffin-embedded tissue sections. This reagent supports studies of epithelial-mesenchymal transition (EMT), tumor invasion, and diagnostic pathology workflows.

Loss of E-cadherin expression is a hallmark of lobular breast carcinoma and diffuse-type gastric carcinoma, making CDH1 immunostaining a useful marker for tumor classification and subtyping. Researchers apply this reagent to characterize cell-cell adhesion in epithelial tissues, screen for hereditary cancer syndromes linked to CDH1 germline mutations, and investigate metastasis mechanisms in cancer biology research.

The unconjugated antibody is supplied as a 200 µl liquid aliquot. Store at -20 °C in working aliquots to preserve reagent stability; avoid repeated freeze-thaw cycles by preparing single-use portions. Host species is mouse with an IgG isotype, and reactivity is confirmed against human tissue, allowing direct use in standard IHC protocols that employ anti-mouse secondary detection systems such as HRP or alkaline phosphatase conjugates.