Anti-CDH1 Antibody

The CDH1 gene encodes E-cadherin, a calcium-dependent transmembrane glycoprotein that mediates homotypic cell-cell adhesion in epithelial tissues. This rabbit polyclonal reagent from Antibodies.com is supplied as 50 µl of unconjugated serum and is validated for western blot and immunohistochemistry on human, mouse, and rat samples, supporting a broad range of epithelial biology and tumor research workflows.

Loss of E-cadherin expression is a hallmark of epithelial-mesenchymal transition, making CDH1 a frequently tracked marker in cancer research, particularly in lobular breast carcinoma and diffuse gastric cancer where its expression is frequently lost. In immunohistochemistry, the antibody highlights the membrane and lateral borders of epithelial cells, while western blot detects the characteristic ~120 kDa band under standard reducing SDS-PAGE conditions. The reactivity profile across human, mouse, and rat enables consistent comparisons between cell-line, xenograft, and primary-tissue experiments.

The unconjugated format allows pairing with user-selected secondary antibodies and labels, accommodating both chromogenic HRP-DAB and fluorescence detection schemes. Aliquot upon first use and store frozen at -20°C; limit freeze-thaw cycles to preserve activity across long-term storage, and protect from light if any fluorescent secondary is later conjugated.