Anti-CDH1 Antibody

E-cadherin, encoded by the CDH1 gene, is a calcium-dependent transmembrane glycoprotein that maintains adherens junctions in epithelial tissue. Antibodies.com offers this rabbit polyclonal reagent for researchers studying epithelial-mesenchymal transition, tumor invasion, and epithelial barrier function. The polyclonal format presents multiple epitope specificities on CDH1, which helps when probing denatured lysates or formalin-fixed paraffin-embedded sections where linear recognition matters more than conformational matching.

The product ships as 100 µl of unconjugated antiserum in a single vial, validated for western blot (WB) and immunohistochemistry (IHC) applications. Cross-species reactivity covers Human, Mouse, and Rat samples, enabling comparative work between patient-derived xenografts, murine knockout models, and rat tissue archives. Recommended storage is at -20 °C in working aliquots, which limits freeze-thaw cycles and preserves binding activity across the reagent's shelf life.

Common use cases include tracking E-cadherin expression in carcinoma cell lines during EMT induction, screening tissue microarrays for epithelial marker loss in invasive tumors, and confirming CRISPR or RNAi knockdown of CDH1 by immunoblotting. The unconjugated format gives laboratories flexibility to pair this primary antibody with their preferred HRP, fluorophore, or biotin secondary reagent for chromogenic or immunofluorescence detection on stained slides and membranes.