Anti-CDH1 Antibody

The CDH1 gene encodes E-cadherin, a calcium-dependent transmembrane glycoprotein that mediates homophilic adhesion between adjacent epithelial cells and anchors adherens junctions to the actin cytoskeleton via β-catenin. This polyclonal reagent from Antibodies.com was raised in rabbit and is supplied unconjugated in a 100 µl vial, suitable for western blot and immunohistochemistry workflows on human and mouse tissue samples.

Loss of CDH1 expression is a hallmark of the epithelial-mesenchymal transition (EMT), and germline mutations in this gene are causally linked to hereditary diffuse gastric cancer (HDGC) and lobular breast carcinoma, making reliable detection of the protein central to epithelial biology and translational cancer research. The antibody cross-reacts with both human and mouse CDH1, supporting comparative studies in cell culture models, tumor tissue microarrays, and developmental systems. On western blot, it detects the full-length ~120 kDa E-cadherin band, while immunohistochemistry applications include evaluating membrane staining intensity in epithelial tumor sections. Store refrigerated in single-use aliquots, protect from light, and avoid repeated freeze-thaw cycles to preserve binding activity.