Anti-CDC45L Antibody

Raised in rabbit against the human CDC45L protein, this unconjugated polyclonal antibody from Antibodies.com targets a conserved factor required for the initiation of eukaryotic DNA replication. CDC45L assembles into the CMG helicase complex alongside MCM2-7 and GINS, and the antibody therefore supports studies of replication origin firing, S-phase entry, and replication fork progression in mammalian cells.

The reagent is supplied as a 100 µl liquid aliquot and is validated for western blot and immunofluorescence workflows, making it appropriate for probing endogenous CDC45L levels in cultured human cell lines and in fixed tissue sections. Host species is rabbit, clonality is polyclonal, and the reagent binds the human target without conjugation, allowing flexibility with secondary reporters chosen by the researcher.

Typical uses include monitoring CDC45L expression across the cell cycle, exploring its recruitment to chromatin during DNA synthesis, and investigating links to replication stress and Meier-Gorlin syndrome, a disorder associated with reduced activity of this replication factor. Refrigerate on arrival and aliquot to avoid repeated freeze-thaw cycles so that antibody activity is preserved across multiple experiments.