Anti-CDC27 Antibody

This unconjugated rabbit polyclonal IgG reagent from Antibodies.com recognizes CDC27, a core scaffold subunit of the anaphase-promoting complex/cyclosome (APC/C) that drives the metaphase-to-anaphase transition and mitotic exit. Supplied as 50 µl of polyclonal antiserum, the antibody is validated for Western blot, immunohistochemistry, and immunofluorescence applications on human, mouse, and rat samples.

CDC27 sits within the APC/C ubiquitin ligase, recruiting substrates such as cyclin B and securin for proteasomal degradation during late mitosis. Anti-CDC27 antibodies are used to monitor chromosome segregation, spindle checkpoint signaling, mitotic progression, and APC/C complex assembly across WB membranes, fixed tissue sections, and cultured cell preparations. The unconjugated format gives labs flexibility to choose secondary antibodies and chromogenic, chemiluminescent, or fluorescent detection chemistries.

Host species is rabbit, clonality polyclonal, isotype IgG, with no fluorophore or enzyme attached. For best results, refrigerate the vial, aliquot upon first use, and limit freeze-thaw cycles to preserve antibody titer. Researchers should empirically titrate the reagent for their specific sample type, fixation protocol, and detection system.