Anti-CDC27 Antibody

This polyclonal reagent from Antibodies.com targets CDC27 (Cell Division Cycle 27), a core subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C) E3 ubiquitin ligase that governs the metaphase-to-anaphase transition and mitotic exit through targeted ubiquitination of securin and cyclin B. Raised in rabbit and supplied as an unconjugated IgG preparation in a 200 µl volume, the antibody recognizes endogenous CDC27 across human, mouse, and rat samples. Verified applications include Western blot for protein-level studies in cell lysates, immunohistochemistry for localization in fixed tissue sections, and immunofluorescence for subcellular distribution, supporting research into cell cycle regulation, spindle assembly checkpoint function, and aneuploidy-related cancer biology, where CDC27 misexpression has been documented in colorectal and other solid tumors.