Anti-CDC26 Antibody

CDC26 is one of the core subunits of the Anaphase-Promoting Complex/Cyclosome (APC/C), a multi-subunit E3 ubiquitin ligase that drives metaphase-to-anaphase transition and exit from mitosis by targeting securin and cyclin B for proteasomal degradation. This rabbit polyclonal reagent from Antibodies.com was raised against recombinant human CDC26 and is supplied as an unconjugated format in a 100 µl volume. It detects the human protein in western blot and immunofluorescence workflows, where it is commonly used to monitor APC/C integrity, study mitotic checkpoint signaling, and probe the assembly state of the complex under perturbations such as spindle poison treatment or subunit knockout. Because CDC26 stabilizes the platform subcomplex of APC/C, loss of signal can indicate misassembly of the holoenzyme, while overexpression studies have linked the subunit to control of the spindle assembly checkpoint in human cells. The polyclonal format provides epitope coverage across multiple regions of the small subunit, supporting detection even when a single linear epitope is masked by binding partners. Researchers working on chromosome segregation, mitotic timing, or anaphase onset in human cell lines will find this reagent useful for confirming subunit incorporation into APC/C purified by immunoprecipitation and for comparing endogenous versus tagged expression levels.