Anti-CDC25C Antibody

This rabbit polyclonal reagent from Antibodies.com targets the cell division cycle 25C dual-specificity phosphatase, the enzyme that removes inhibitory phosphates from the cyclin B/CDK1 complex and drives the G2/M transition of the eukaryotic cell cycle. The antibody is supplied as 100 µl of unconjugated, ready-to-use reagent in a vial and has been validated for Western blot, immunohistochemistry, immunofluorescence, and immunoprecipitation workflows, with reactivity confirmed against human, mouse, and rat samples.

CDC25C sits at the centre of the mitotic entry checkpoint. Following DNA damage, the sensor kinases ATR and CHK1 phosphorylate CDC25C at serine 216, generating a 14-3-3 binding site that sequesters the phosphatase in the cytoplasm and blocks premature mitotic commitment. Loss of this regulatory layer is a frequent feature of transformed cells and is widely exploited in oncology research, where CDC25C expression and phosphorylation status are scored as markers of checkpoint integrity, mitotic timing, and cellular response to genotoxic stress.

The polyclonal host response yields an antibody pool that recognises multiple epitopes on the target, which is useful for native immunoprecipitation captures and helps tolerate minor sample-to-sample variation in IHC and IF staining. The unconjugated format leaves the choice of secondary antibody and detection chemistry to the end user. Keep refrigerated; aliquot upon first thaw if extended storage is intended, and avoid repeated freeze-thaw cycles to preserve binding activity.