Anti-Cdc25C Antibody

This monoclonal antibody is raised in mouse against Cdc25C, a dual-specificity phosphatase that removes inhibitory phosphates from the CDK1/cyclin B complex and gates the G2/M transition. Distributed by Antibodies.com as 100 µL of ascitic fluid containing 0.03% sodium azide as preservative, the unconjugated reagent is validated for western blot detection of the human target. Cdc25C is a focal point for cell cycle and DNA damage response research: checkpoint kinases Chk1 and Chk2 phosphorylate the phosphatase on Ser216, promoting binding of 14-3-3 proteins and cytoplasmic sequestration, while checkpoint recovery requires reactivation to permit mitotic entry. Researchers tracking mitotic progression, studying checkpoint abrogation following genotoxic stress, or profiling proliferation in cancer cell line panels will find this Mouse-host primary useful. The unconjugated format is compatible with HRP- or fluorophore-conjugated secondary antibodies, supporting chemiluminescent or fluorescent western blot readout of Cdc25C levels across asynchronous, synchronized, or drug-treated cell populations.