Anti-Cdc25A (phospho-S76) Antibody

Cdc25A phosphatase activity is tightly controlled through site-specific phosphorylation, and serine 76 represents a regulatory site linked to protein turnover and DNA damage checkpoint signaling. This rabbit polyclonal reagent from Antibodies.com was raised against the phospho-S76 epitope, allowing selective detection of the phosphorylated Cdc25A pool in human, mouse, and rat samples by Western blot or immunohistochemistry workflows.

The antibody is supplied as 100 µl of purified immunoglobulin at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide, at approximately pH 7.2. As an unconjugated polyclonal reagent, it can be paired with secondary antibodies of the researcher's choice for chromogenic, chemiluminescent, or fluorescent detection. Typical applications include monitoring pathway activation following genotoxic stress, assessing checkpoint fidelity in tumor models, profiling G1/S and G2/M transition events, and distinguishing the regulated phosphatase pool from total Cdc25A expression in fixed tissue sections.

For research use only. Refrigerate the vial on receipt and protect from light; aliquot to minimize repeated freeze-thaw cycles and preserve antibody activity. Each lot is validated across the listed species reactivities to support reproducible cell cycle and DNA damage response studies.