Anti-Cdc25A Antibody

This rabbit polyclonal antibody from Antibodies.com targets Cdc25A, a dual-specificity phosphatase that activates cyclin-dependent kinases (CDKs) by removing inhibitory phosphates from tyrosine and threonine residues. Cdc25A drives the G1/S phase transition, licenses DNA replication origin firing, and contributes to the S-phase checkpoint, with documented roles in DNA damage signaling and recovery from genotoxic stress.

Cdc25A overexpression and gene amplification have been reported in multiple human cancers, including breast, liver, and head and neck tumors, making the protein a long-standing focus in cell cycle biology, oncology, and checkpoint kinase research. The unconjugated polyclonal reagent is supplied in a 100 µl volume and is validated for Western blot (WB) and ELISA workflows across human, mouse, and rat samples, supporting comparative studies in cell lysates, tissue extracts, and recombinant protein preparations.

For Western blot, the antibody can be paired with standard HRP- or fluorophore-conjugated secondary antibodies to detect endogenous Cdc25A, which migrates in the 55–65 kDa range on SDS-PAGE depending on isoform and post-translational modifications. ELISA workflows benefit from the polyclonal format, which recognizes multiple epitopes across the Cdc25A phosphatase domain. The reagent is intended for research use only and should be stored at -20 °C in working aliquots to limit freeze-thaw cycles and preserve binding activity.