Anti-CDC25A Antibody

Manufactured by Antibodies.com as a primary antibody reagent for cell-cycle and oncology research, this polyclonal preparation was generated in rabbit against a recombinant human CDC25A immunogen and affinity-purified to detect the endogenous protein in routine Western blot and immunohistochemistry workflows. The unconjugated reagent is supplied at a concentration of 1.0 mg/mL in a 50 µL aliquot, formulated in phosphate-buffered saline (pH 7.4) containing 150 mM sodium chloride, 0.02% sodium azide, and 50% glycerol, and should be stored at −20 °C while avoiding repeated freeze/thaw cycles. Verified reactivity spans human, mouse, and rat samples, making the antibody suitable for comparative studies across mammalian model systems and primary tissue specimens. Researchers will appreciate the broad application profile, which covers standard immunoblotting of total CDC25A levels as well as IHC detection in fixed sections, while the polyclonal nature of the reagent increases the likelihood of recognising multiple epitopes on the dual-specificity phosphatase. The antibody detects the endogenous level of total CDC25A protein, providing a reliable marker for examining G1/S checkpoint regulation, DNA-damage response signalling, and proliferation status in both cell-line and primary-tissue models. Each lot is intended for research use only and is not validated for diagnostic or therapeutic applications, and the product ships with the standard Antibodies.com handling documentation.