Anti-CDC25A Antibody

The Anti-CDC25A Antibody offered by Antibodies.com is a rabbit polyclonal reagent raised against the cell division cycle 25A phosphatase, a dual-specificity phosphatase that dephosphorylates cyclin-dependent kinases (CDKs) to drive the G1/S transition of the cell cycle. Supplied as an unconjugated, 100 µl aliquot, the antibody is validated for Western blot (WB) and immunohistochemistry (IHC) workflows and cross-reacts with Human, Mouse, and Rat samples, supporting comparative studies across standard laboratory models.

CDC25A is a focal point of the DNA damage response, where ATM/ATR-mediated signaling targets the phosphatase for ubiquitin-dependent degradation to halt cell cycle progression. Because CDC25A is frequently overexpressed or stabilized in multiple tumor types, the antibody is widely used in cancer research to examine checkpoint integrity, proliferation kinetics, and oncogenic signaling. The polyclonal host response allows recognition of multiple epitopes on CDC25A, which can strengthen signal detection in lysate-based assays and tissue sections.

For best results, aliquot the reagent upon first use and store at -20 °C in a frost-free freezer; avoid repeated freeze-thaw cycles to preserve binding activity. Brief refrigeration at 4 °C is appropriate for working dilutions prepared for short-term use. Each vial is intended for research use only and is not validated for diagnostic or therapeutic applications.