Anti-CDC25A Antibody

Produced in rabbit as an unconjugated polyclonal IgG and supplied by Antibodies.com in a 100 µl liquid format, this CDC25A reagent is intended for Western blot detection of endogenous cell division cycle 25A phosphatase across human, mouse, and rat samples. The target protein is a dual-specificity phosphatase that removes inhibitory phosphates from CDK2/Cyclin E and CDK2/Cyclin A complexes to drive G1/S transition and also contributes to G2/M checkpoint control, making the antibody a frequent tool in studies of proliferation, DNA damage response, and oncogenesis where CDC25A overexpression or aberrant regulation is implicated.

The antibody is raised against a synthetic peptide derived from the human CDC25A sequence and recognizes the full-length phosphatase at its predicted molecular weight of approximately 59 kDa. Recommended Western blot loading is in the 1:500 to 1:1000 dilution range, and the unconjugated IgG format leaves the choice of reporter and secondary label to the researcher, supporting straightforward pairing with HRP, fluorophore, or biotin conjugates depending on the detection platform. The polyclonal nature of the reagent provides recognition of multiple epitopes on CDC25A, which can help track shifts in protein abundance or post-translational modification state across experimental conditions.

Researchers commonly apply this reagent to comparative studies across tumor-derived cell lines, primary cultures, and rodent model systems, since cross-reactivity with mouse and rat orthologs allows side-by-side evaluation of CDC25A regulation in transformed versus normal tissue contexts. Storage is recommended at -20°C with aliquoting to limit freeze/thaw cycles, and the reagent is labeled for research use only.