Anti-CDC20 Antibody

Rabbit Polyclonal Antibody to CDC20 for Cell Cycle Research

This polyclonal reagent from Antibodies.com is raised in rabbit and is directed against CDC20 (Cell Division Cycle 20), a WD40-repeat activator of the anaphase-promoting complex/cyclosome (APC/C). CDC20 recruits substrates such as securin and cyclin B1 to the APC/C ubiquitin ligase, driving the metaphase-to-anaphase transition and mitotic exit. Altered CDC20 expression has been reported in a range of tumor types, making the protein a frequent target in cancer cell biology, spindle assembly checkpoint studies, and investigations of chromosomal instability.

The antibody is supplied as an unconjugated preparation in a 100 µl volume. It cross-reacts with human, mouse, and rat samples, supporting comparative work across established cell line and tissue models. Validated applications include Western blot for expression profiling, immunohistochemistry for localization in fixed tissue sections, and immunofluorescence for subcellular distribution during mitosis.

Recommended storage is at -20 °C in working aliquots to limit freeze-thaw cycles, with brief refrigeration for short-term use. The polyclonal format provides recognition of multiple epitopes on CDC20, which is useful when probing full-length protein and its regulated forms. Researchers studying APC/C regulation, mitotic checkpoint fidelity, or mitotic slippage in response to anti-mitotic agents will find this reagent applicable to a range of biochemical and cell-based assays, including investigations of securin degradation, cyclin B1 turnover, and faithful chromosome segregation.