Anti-Cdc2 Antibody

Raised in rabbit, this unconjugated polyclonal antibody from Antibodies.com targets Cdc2 (also known as CDK1), the cyclin-dependent kinase that drives the G2/M transition and mitotic entry in dividing cells. It recognizes the conserved kinase domain and is validated for Western blot and ELISA workflows, with reactivity confirmed against human, mouse, and rat samples.

Each vial contains 100 µL of liquid antiserum, supplied unconjugated so it can be paired with a secondary reporter of the researcher's choice (HRP, fluorophore, or biotin). The polyclonal format provides coverage of multiple epitopes on the Cdc2 target, which is useful when probing denatured lysates or when phospho-state-independent detection is required.

Suggested uses include monitoring cell-cycle progression in synchronized cultures, tracking mitotic entry after checkpoint perturbation, and supporting pull-down or co-immunoprecipitation experiments aimed at characterizing cyclin B and Cdc25 interactions. Store refrigerated at 2 to 8 degrees C; aliquot to limit freeze-thaw cycles.