Anti-CDC2 (Ab-161) Antibody

CDC2 (also known as CDK1) is a cyclin-dependent kinase that plays a central role in cell cycle progression, particularly the G1/S and G2/M transitions through association with cyclin A and cyclin B. This rabbit polyclonal reagent from Antibodies.com is raised against a peptide epitope centered on residue 161 of human CDC2, producing an affinity-purified preparation for detecting endogenous protein levels in research samples.

The unconjugated antibody is supplied as 100 µl of liquid stock and has been validated for Western blot and immunofluorescence workflows across human, mouse, and rat samples. The polyclonal format increases the likelihood of recognizing multiple epitopes on CDC2, which can be advantageous when monitoring expression changes or phosphorylation shifts during cell cycle studies. Researchers studying mitotic entry, checkpoint control, or cancer cell proliferation commonly use anti-CDC2 antibodies to probe lysates prepared from synchronized cultures, tumor-derived cell lines, and tissue sections.

Storage at 4 °C is recommended for short-term use, with aliquoting advised to limit freeze-thaw cycles and preserve antibody activity over the working shelf life. For immunofluorescence, the reagent is often applied to fixed cells to visualize centrosomal and nuclear staining patterns during mitosis.