Anti-CD95/FAS (C-terminus) Antibody

This mouse monoclonal antibody from Antibodies.com specifically recognizes the C-terminal region of human CD95, also known as FAS or APO-1, a type I transmembrane receptor that initiates the extrinsic apoptotic signaling cascade upon engagement by FAS ligand. The unconjugated reagent is supplied as 100 µL of purified immunoglobulin in a stabilizing buffer composed of 0.1 M Tris-Glycine at pH 7.4 with 150 mM NaCl, 50% glycerol, and 0.2% sodium azide as a preservative. Validated for Western blot detection, the antibody binds the intracellular C-terminus of CD95, supporting studies of death receptor expression, DISC assembly, and caspase-8 recruitment in human cell lines and tissue lysates. Researchers working on chemotherapy-induced apoptosis, autoimmune lymphoproliferative syndrome, immune privilege, and tumor immune evasion rely on C-terminal CD95 reagents to distinguish full-length receptor from cleaved or truncated isoforms that arise during programmed cell death. Storage at -20 °C in single-use aliquots preserves activity through repeated freeze-thaw cycles, and the Tris-Glycine formulation is compatible with standard SDS-PAGE workflows without further dilution before blotting.