Anti-CD86 Antibody [Assigned #A10795]

Recognising the 70 kDa costimulatory glycoprotein CD86 (B7-2), this unconjugated rabbit monoclonal IgG reagent from Antibodies.com is validated for Western blot, immunohistochemistry, immunofluorescence, and immunoprecipitation workflows in human and rat samples. CD86 sits on the surface of antigen-presenting cells such as dendritic cells, activated B lymphocytes, and macrophages, where its engagement of CD28 and CTLA-4 delivers the second signal required for full T cell activation and shapes peripheral tolerance, making it a central marker in studies of costimulation, checkpoint biology, and APC phenotype.

On Western blots the antibody detects the characteristic 70 kDa band of full-length CD86; on fixed tissue sections and cultured cells it produces clean membrane-associated staining compatible with standard anti-rabbit IgG secondary detection systems; and in immunoprecipitation workflows it readily pulls native CD86-containing complexes from primary lysates. The rabbit monoclonal format reduces lot-to-lot variability compared with polyclonal reagents, while the unconjugated format leaves the antibody open to custom fluorophore, biotin, or enzyme labelling when a directly conjugated primary is not available.

Typical applications include probing how CD86 surface expression changes upon APC activation, characterising costimulatory ligand profiles in transplant and autoimmune disease models, isolating receptor complexes from primary cells, and confirming CD86 distribution across human and rat tissue specimens. Supplied as 100 µl of stock reagent, the antibody slots directly into established immunostaining, blotting, and immunoprecipitation protocols without further dilution.