Anti-CD8 Antibody [JXYT8]

From Antibodies.com, this lyophilised rat monoclonal reagent targets the CD8α co-receptor expressed on mouse cytotoxic T lymphocytes, supplying 100 µg of purified antibody in the JXYT8 clone. The IgM isotype sets this reagent apart from the more common IgG anti-CD8 antibodies, lending itself to studies that require an isotype-controlled background or that leverage IgM-specific secondary reagents. Researchers apply it to flow cytometry of splenocyte and lymph node preparations, immunohistochemistry on frozen tissue sections, and in vivo depletion experiments where selective removal of CD8+ cells is required before adoptive transfer or pathogen challenge. The unconjugated format supports site-specific labelling with biotin, fluorophores such as FITC or PE, or enzymes depending on the downstream assay; reconstitute the lyophilised pellet in sterile buffer and aliquot to limit repeated freeze-thaw exposure of working stocks. Stored refrigerated for short-term use, the antibody retains activity through long-term frozen storage when handled under sterile conditions, and its reactivity is directed at the mouse CD8α orthologue as recorded in the product specifications.